9I62
CryoEM structure of a RAD51 D-loop
Summary for 9I62
| Entry DOI | 10.2210/pdb9i62/pdb |
| EMDB information | 52646 |
| Descriptor | DNA repair protein RAD51 homolog 1, DNA (26-MER), DNA (41-MER), ... (6 entities in total) |
| Functional Keywords | dna repair, homologous recombination, strand exchange, atpase, dna binding protein |
| Biological source | Homo sapiens (human) More |
| Total number of polymer chains | 12 |
| Total formula weight | 378883.11 |
| Authors | Pellegrini, L.,Joudeh, L.,Appleby, R.E. (deposition date: 2025-01-29, release date: 2025-06-04, Last modification date: 2025-09-03) |
| Primary citation | Joudeh, L.,Appleby, R.E.,Maman, J.D.,Pellegrini, L. Structural mechanism of strand exchange by the RAD51 filament. Elife, 14:-, 2025 Cited by PubMed Abstract: Homologous recombination (HR) preserves genomic stability by repairing double-strand DNA breaks and ensuring efficient DNA replication. Central to HR is the strand-exchange reaction taking place within the three-stranded synapsis wherein a RAD51 nucleoprotein filament binds to a donor DNA. Here, we present the cryoEM structure of a displacement loop of human RAD51 that captures the synaptic state when the filament has become tightly bound to the donor DNA. The structure elucidates the mechanism of strand exchange by RAD51, including the filament engagement with the donor DNA, the strand invasion and pairing with the complementary sequence of the donor DNA, the capture of the non-complementary strand and the polarity of the strand-exchange reaction. Our findings provide fundamental mechanistic insights into the biochemical reaction of eukaryotic HR. PubMed: 40820711DOI: 10.7554/eLife.107114 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.64 Å) |
Structure validation
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