9HSA
Solution structure of X55, a computationally designed protein
Summary for 9HSA
| Entry DOI | 10.2210/pdb9hsa/pdb |
| Descriptor | X55 (1 entity in total) |
| Functional Keywords | computational design, de novo protein |
| Biological source | Escherichia coli |
| Total number of polymer chains | 1 |
| Total formula weight | 12037.83 |
| Authors | Schweimer, K.,Hennig, J.,Perez-Borrajero, C. (deposition date: 2024-12-19, release date: 2025-04-23, Last modification date: 2025-09-17) |
| Primary citation | Jendrusch, M.A.,Yang, A.L.J.,Cacace, E.,Bobonis, J.,Voogdt, C.G.P.,Kaspar, S.,Schweimer, K.,Perez-Borrajero, C.,Lapouge, K.,Scheurich, J.,Remans, K.,Hennig, J.,Typas, A.,Korbel, J.O.,Sadiq, S.K. AlphaDesign: a de novo protein design framework based on AlphaFold. Mol.Syst.Biol., 21:1166-1189, 2025 Cited by PubMed Abstract: De novo protein design is of fundamental interest to synthetic biology, with a plethora of computational methods of various degrees of generality developed in recent years. Here, we introduce AlphaDesign, a hallucination-based computational framework for de novo protein design developed with maximum generality and usability in mind, which combines AlphaFold with autoregressive diffusion models to enable rapid generation and computational validation of proteins with controllable interactions, conformations and oligomeric state without the requirement for class-dependent model re-training or fine-tuning. We apply our framework to design and systematically validate in vivo active inhibitors of a family of bacterial phage defense systems with toxic effectors called retrons, paving the way towards efficient, rational design of novel proteins as biologics. PubMed: 40527958DOI: 10.1038/s44320-025-00119-z PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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