9HCJ

D. discoideum nuclear pore complex

This is a non-PDB format compatible entry.

Summary for 9HCJ

• Entry DOI: 10.2210/pdb9hcj/pdb
• EMDB information: 19137
• Descriptor: RanBP2-type zinc finger, Nuclear pore complex protein nup54, Nup58, ... (25 entities in total)
• Functional Keywords: nuclear pore complex, nuclear protein
• Biological source: Dictyostelium discoideum; More
• Total number of polymer chains: 96
• Total formula weight: 11895812.16

Authors

Obarska-Kosinska, A.,Hoffmann, P.C.,Beck, M. (deposition date: 2024-11-10, release date: 2024-12-25, Last modification date: 2025-02-19)

Primary citation

Hoffmann, P.C.,Kim, H.,Obarska-Kosinska, A.,Kreysing, J.P.,Andino-Frydman, E.,Cruz-Leon, S.,Margiotta, E.,Cernikova, L.,Kosinski, J.,Turonova, B.,Hummer, G.,Beck, M.
Nuclear pore permeability and fluid flow are modulated by its dilation state.
Mol.Cell, 85:537-, 2025

PubMed Abstract: Changing environmental conditions necessitate rapid adaptation of cytoplasmic and nuclear volumes. We use the slime mold Dictyostelium discoideum, known for its ability to tolerate extreme changes in osmolarity, to assess which role nuclear pore complexes (NPCs) play in achieving nuclear volume adaptation and relieving mechanical stress. We capitalize on the unique properties of D. discoideum to quantify fluid flow across NPCs. D. discoideum has an elaborate NPC structure in situ. Its dilation state affects NPC permeability for nucleocytosolic flow. Based on mathematical concepts adapted from hydrodynamics, we conceptualize this phenomenon as porous flow across NPCs, which is distinct from canonically characterized modes of nucleocytoplasmic transport because of its dependence on pressure. Viral NPC blockage decreased nucleocytosolic flow. Our results may be relevant for any biological conditions that entail rapid nuclear size adaptation, including metastasizing cancer cells, migrating cells, or differentiating tissues.

PubMed: 39729993
DOI: 10.1016/j.molcel.2024.11.038

Experimental method

ELECTRON MICROSCOPY (30 Å)