9GYI
Cryo-EM structure of the capsid-forming phage-inducible chromosomal island (cf-PICI) EcCI2
Summary for 9GYI
| Entry DOI | 10.2210/pdb9gyi/pdb |
| EMDB information | 51699 |
| Descriptor | Major head protein (1 entity in total) |
| Functional Keywords | pici, cf-pici, bacteriophage, capsid, phage-inducible chromosomal island, virus like particle |
| Biological source | Escherichia coli O157:H7 str. EDL933 |
| Total number of polymer chains | 4 |
| Total formula weight | 172701.72 |
| Authors | Patkowski, J.B.,Penades, J.R.,Costa, T.R.D. (deposition date: 2024-10-02, release date: 2025-09-10, Last modification date: 2025-11-26) |
| Primary citation | He, L.,Patkowski, J.B.,Wang, J.,Miguel-Romero, L.,Aylett, C.H.S.,Fillol-Salom, A.,Costa, T.R.D.,Penades, J.R. Chimeric infective particles expand species boundaries in phage-inducible chromosomal island mobilization. Cell, 188:6636-6653.e17, 2025 Cited by PubMed Abstract: Some mobile genetic elements spread among unrelated bacterial species through unknown mechanisms. Recently, we discovered that identical capsid-forming phage-inducible chromosomal islands (cf-PICIs), a new family of phage satellites, are present across multiple species and genera, raising questions about their widespread dissemination. Here, we have identified and characterized a new biological entity enabling this transfer. Unlike other satellites, cf-PICIs produce their own capsids and package their DNA, relying solely on phage tails for transfer. cf-PICIs release non-infective, tailless capsids containing their DNA into the environment. These subcellular entities then interact with phage tails from various species, forming chimeric particles that inject DNA into different bacterial species depending on the tail present. Additionally, we elucidated the structure of the tailless cf-PICIs and the mechanism behind their unique capsid formation. Our findings illuminate the mechanisms used by satellites to spread in nature, contributing to bacterial evolution and the emergence of new pathogens. PubMed: 40930093DOI: 10.1016/j.cell.2025.08.019 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.24 Å) |
Structure validation
Download full validation report
