9GVG
P116 from Mycoplasma pneumoniae in complex with mild growth suppressor monoclonal antibody
This is a non-PDB format compatible entry.
Summary for 9GVG
| Entry DOI | 10.2210/pdb9gvg/pdb |
| EMDB information | 51635 |
| Descriptor | Heavy chain monoclonal antibody, Light chain monoclonal antibody, Uncharacterized protein MG075 homolog (3 entities in total) |
| Functional Keywords | immunodominant, cholesterol, essential lipids, transporter, lipid binder, monoclonal antibody, protein transport |
| Biological source | Mus musculus More |
| Total number of polymer chains | 4 |
| Total formula weight | 228509.07 |
| Authors | Vizarraga, D.,Marcos Silva, M.,Martin Romero, J.,Guerra, P.,Fita, I.,Pinyol, J. (deposition date: 2024-09-24, release date: 2025-12-24) |
| Primary citation | Vizarraga, D.,Marcos, M.,Rotllan, N.,Martin, J.,Santos, D.,Camacho, M.,Soto, B.,Velasco-Reniu, L.,Guerra, P.,Pareja, F.,Collantes, M.,Wu, W.,Rodriguez-Arce, I.,Serrano, L.,Pinol, J.,Fita, I.,Escola-Gil, J.C. Sources of essential lipids for Mycoplasma pneumoniae via P116 to target liver and atherosclerotic lesions. Nat Commun, 16:11159-11159, 2025 Cited by PubMed Abstract: Mycoplasma pneumoniae (MPN) is a bacterial pathogen that primarily causes atypical pneumonia. It cannot synthesize certain essential lipids and therefore relies on the host for their acquisition to survive. MPN has been detected in increased amounts within ruptured atherosclerotic plaques. In this work, we show that the protein P116 facilitates cholesterol acquisition from LDL, HDL and various cell types. Targeting P116's C-terminal domain with a monoclonal antibody inhibits cholesterol acquisition and bacterial growth in vitro. Phase contrast epifluorescence microscopy of human arteries reveals that this antibody blocks MPN binding to atherosclerotic lesions ex vivo. Additionally, an MPN chassis injected into hyperlipidemic female mice localizes to the liver and atherosclerotic plaques. Here, we report that P116 plays a role in extracting essential lipids from lipoproteins and host cells and regulates MPN localization to atheromatous plaques. The study highlights MPN's potential as a tool for targeting atherosclerotic lesions and fatty liver. PubMed: 41402265DOI: 10.1038/s41467-025-66129-5 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.06 Å) |
Structure validation
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