9GV5
Human monocarboxylate transporter 8 D424N mutant
Summary for 9GV5
| Entry DOI | 10.2210/pdb9gv5/pdb |
| EMDB information | 51624 |
| Descriptor | Monocarboxylate transporter 8 (1 entity in total) |
| Functional Keywords | thyroid, thyroid hormones, transporter, allan-herndon-dudley syndrome, transport protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 57774.90 |
| Authors | Tassinari, M.,Coscia, F. (deposition date: 2024-09-22, release date: 2025-05-21, Last modification date: 2025-06-11) |
| Primary citation | Tassinari, M.,Tanzi, G.,Maggiore, F.,Groeneweg, S.,van Geest, F.S.,Freund, M.E.T.,Stavast, C.J.,Boniardi, I.,Pasqualato, S.,Visser, W.E.,Coscia, F. Molecular mechanism of thyroxine transport by monocarboxylate transporters. Nat Commun, 16:4493-4493, 2025 Cited by PubMed Abstract: Thyroid hormones (the common name for prohormone thyroxine and the bioactive form triiodothyronine) control major developmental and metabolic processes. Release of thyroid hormones from the thyroid gland into the bloodstream and their transport into target cells is facilitated by plasma membrane transporters, including monocarboxylate transporter (MCT)8 and the highly homologous MCT10. However, the molecular mechanism underlying thyroid hormone transport is unknown. The relevance of such transporters is illustrated in patients with MCT8 deficiency, a severe neurodevelopmental and metabolic disorder. Using cryogenic-sample electron microscopy (cryo-EM), we determined the ligand-free and thyroxine-bound human MCT8 structures in the outward-facing state and the thyroxine-bound human MCT10 in the inward-facing state. Our structural analysis revealed a network of conserved gate residues involved in conformational changes upon thyroxine binding, triggering ligand release in the opposite compartment. We then determined the structure of a folded but inactive patient-derived MCT8 mutant, indicating a subtle conformational change which explains its reduced transport activity. Finally, we report a structure of MCT8 bound to its inhibitor silychristin, locked in the outward-facing state, revealing the molecular basis of its action and specificity. Taken together, this study advances mechanistic understanding of normal and disordered thyroid hormone transport. PubMed: 40368961DOI: 10.1038/s41467-025-59751-w PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4 Å) |
Structure validation
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