9GUS
30S mRNA delivery complex TEC resolved (30S only)
Summary for 9GUS
| Entry DOI | 10.2210/pdb9gus/pdb |
| Related | 9GUR |
| EMDB information | 51618 |
| Descriptor | 16S ribosomal RNA, 30S ribosomal protein S9, 30S ribosomal protein S10, ... (25 entities in total) |
| Functional Keywords | transcription, translation, coupling., ribosome |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 24 |
| Total formula weight | 891436.90 |
| Authors | Rahil, H.,Weixlbaumer, A.,Webster, M.W. (deposition date: 2024-09-20, release date: 2024-11-20, Last modification date: 2024-12-11) |
| Primary citation | Webster, M.W.,Chauvier, A.,Rahil, H.,Graziadei, A.,Charles, K.,Miropolskaya, N.,Takacs, M.,Saint-Andre, C.,Rappsilber, J.,Walter, N.G.,Weixlbaumer, A. Molecular basis of mRNA delivery to the bacterial ribosome. Science, 386:eado8476-eado8476, 2024 Cited by PubMed Abstract: Protein synthesis begins with the formation of a ribosome-messenger RNA (mRNA) complex. In bacteria, the small ribosomal subunit (30) is recruited to many mRNAs through base pairing with the Shine-Dalgarno (SD) sequence and RNA binding by ribosomal protein bS1. Translation can initiate on nascent mRNAs, and RNA polymerase (RNAP) can promote the recruitment of the pioneering 30. Here, we examined 30 recruitment to nascent mRNAs using cryo-electron microscopy, single-molecule fluorescence colocalization, and in-cell cross-linking mass spectrometry. We show that bS1 delivers the mRNA to the ribosome for SD duplex formation and 30 activation. Additionally, bS1 and RNAP stimulate translation initiation. Our work provides a mechanistic framework for how the SD duplex, ribosomal proteins, and RNAP cooperate in 30 recruitment to mRNAs and establish transcription-translation coupling. PubMed: 39607923DOI: 10.1126/science.ado8476 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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