9GOS
CryoEM structure of the native Chlamydomonas reinhardtii Flagella Membrane Glycoprotein 1B.
Summary for 9GOS
| Entry DOI | 10.2210/pdb9gos/pdb |
| EMDB information | 51499 |
| Descriptor | Flagella Membrane Glycoprotein 1B (1 entity in total) |
| Functional Keywords | mucin, glycoprotein, glycocalyx, membrane protein |
| Biological source | Chlamydomonas reinhardtii |
| Total number of polymer chains | 1 |
| Total formula weight | 448226.63 |
| Authors | Nievergelt, A.P.,Hoepfner, L.M.,Matrino, F.,Scholz, M.,Foster, H.E.,Rodenfels, J.,von Appen, A.,Hippler, M.,Pigino, G. (deposition date: 2024-09-06, release date: 2025-05-07, Last modification date: 2025-11-19) |
| Primary citation | Hoepfner, L.M.,Nievergelt, A.P.,Matrino, F.,Scholz, M.,Foster, H.E.,Rodenfels, J.,von Appen, A.,Hippler, M.,Pigino, G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci, 12:e2413355-e2413355, 2025 Cited by PubMed Abstract: The glycocalyx, a highly heterogeneous glycoprotein layer of cilia regulates adhesion and force transduction and is involved in signaling. The high-resolution molecular architecture of this layer is currently not understood. The structure of the ciliary coat is described in the green alga Chlamydomonas reinhardtii by cryo-electron tomography and proteomic approaches and the high-resolution cryoEM structure of the main component, FMG1B is solved. FMG1B is described as a mucin orthologue which lacks the major O-glycosylation of mammalian mucins but is N-glycosylated. FMG1A, a previously undescribed isoform of FMG1B is expressed in C. reinhardtii. By microflow-based adhesion assays, increased surface adhesion in the glycocalyx deficient double-mutant fmg1b-fmg1a is observed. It is found this mutant is capable of surface-gliding, with neither isoform required for extracellular force transduction by intraflagellar transport. The results find FMG1 to form a protective layer with adhesion-regulative instead of adhesion-conferring properties and an example of an undescribed class of mucins. PubMed: 40041987DOI: 10.1002/advs.202413355 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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