9GLC

NONO/SFPQ filament: local refinement central units (strand 1)

これはPDB形式変換不可エントリーです。

9GLC の概要

• エントリーDOI: 10.2210/pdb9glc/pdb
• 関連するPDBエントリー: 9GLD 9GNI
• EMDBエントリー: 51438 51471
• 分子名称: Splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated), Non-POU domain containing octamer binding (2 entities in total)
• 機能のキーワード: filament, rna binding, dna binding, gene regulation, nuclear protein
• 由来する生物種: Cricetulus griseus (Chinese hamster); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 673360.25

構造登録者

Rasmussen, T.,Bottcher, B. (登録日: 2024-08-27, 公開日: 2025-09-10, 最終更新日: 2026-03-11)

主引用文献

Rasmussen, T.,Kuspert, J.,Schonemann, L.,Geiger, D.,Bottcher, B.
The gene-regulating proteins NONO and SFPQ assemble into ordered filaments.
Commun Biol, 9:117-117, 2025

PubMed Abstract: Proteins of the Drosophila behaviour/human splicing (DBHS) family are involved in many aspects of gene regulation and maintenance like transcription, splicing and DNA repair. DBHS proteins form obligate homo- and heterodimers through interactions within a globular domain and can further dynamically oligomerise through α-helical coiled-coils, which is crucial for many functions. While the atomic structures of the dimers are established, the arrangement in higher oligomers is unknown. Here we present the structure of a filamentous NONO/SFPQ heterooligomer resolved by cryo-EM. The filaments form a double helix which is stabilized by an interdigitating network of coiled-coil interactions.

PubMed: 41476260
DOI: 10.1038/s42003-025-09396-8

実験手法

ELECTRON MICROSCOPY (3.3 Å)