9GGN

Cryo-EM structure of KBTBD4 WT-HDAC2 2:2 complex mediated by molecular glue UM171

これはPDB形式変換不可エントリーです。

9GGN の概要

• エントリーDOI: 10.2210/pdb9ggn/pdb
• EMDBエントリー: 51338
• 分子名称: Isoform 1 of Kelch repeat and BTB domain-containing protein 4, Histone deacetylase 2, (1r,4r)-N~1~-[(7P)-2-benzyl-7-(2-methyl-2H-tetrazol-5-yl)-9H-pyrimido[4,5-b]indol-4-yl]cyclohexane-1,4-diamine, ... (4 entities in total)
• 機能のキーワード: e3 ligase, deacetylase, ubiquitylation, transcription, molecular glue, complex, ligase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 228341.70

構造登録者

Chen, Z.,Chi, G.,Pike, A.C.W.,Montes, B.,Bullock, A.N. (登録日: 2024-08-13, 公開日: 2025-04-09)

主引用文献

Chen, Z.,Chi, G.,Balo, T.,Chen, X.,Montes, B.R.,Clifford, S.C.,D'Angiolella, V.,Szabo, T.,Kiss, A.,Novak, T.,Herner, A.,Kotschy, A.,Bullock, A.N.
Structural mimicry of UM171 and neomorphic cancer mutants co-opts E3 ligase KBTBD4 for HDAC1/2 recruitment.
Nat Commun, 16:3144-3144, 2025

PubMed Abstract: Neomorphic mutations and drugs can elicit unanticipated effects that require mechanistic understanding to inform clinical practice. Recurrent indel mutations in the Kelch domain of the KBTBD4 E3 ligase rewire epigenetic programs for stemness in medulloblastoma by recruiting LSD1-CoREST-HDAC1/2 complexes as neo-substrates for ubiquitination and degradation. UM171, an investigational drug for haematopoietic stem cell transplantation, was found to degrade LSD1-CoREST-HDAC1/2 complexes in a wild-type KBTBD4-dependent manner, suggesting a potential common mode of action. Here, we identify that these neomorphic interactions are mediated by the HDAC deacetylase domain. Cryo-EM studies of both wild-type and mutant KBTBD4 capture 2:1 and 2:2 KBTBD4-HDAC2 complexes, as well as a 2:1:1 KBTBD4-HDAC2-CoREST1 complex, at resolutions spanning 2.7 to 3.3 Å. The mutant and drug-induced complexes adopt similar structural assemblies requiring both Kelch domains in the KBTBD4 dimer for each HDAC2 interaction. UM171 is identified as a bona fide molecular glue binding across the ternary interface. Most strikingly, the indel mutation reshapes the same surface of KBTBD4 providing an example of a natural mimic of a molecular glue. Together, the structures provide mechanistic understanding of neomorphic KBTBD4, while structure-activity relationship (SAR) analysis of UM171 reveals analog S234984 as a more potent molecular glue for future studies.

PubMed: 40175372
DOI: 10.1038/s41467-025-58350-z

実験手法

ELECTRON MICROSCOPY (2.9 Å)