9G0A

Influenza A/H7N9 polymerase post-cleavage cap-snatching complex

9G0A の概要

• エントリーDOI: 10.2210/pdb9g0a/pdb
• 関連するPDBエントリー: 9FYX
• EMDBエントリー: 50927
• 分子名称: Transcription elongation factor SPT5, likely linker between KOWx4 and KOW5 domains of SPT5, DNA-directed RNA polymerase subunit beta, ... (27 entities in total)
• 機能のキーワード: influenza virus, influenza a, cap-snatching, transcription, virus
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 926849.57

構造登録者

Rotsch, A.H.,Li, D.,Dienemann, C.,Cusack, S.,Cramer, P. (登録日: 2024-07-07, 公開日: 2025-07-16, 最終更新日: 2026-03-11)

主引用文献

Rotsch, A.H.,Li, D.,Dupont, M.,Krischuns, T.,Neef, U.,Oberthur, C.,Stelfox, A.,Lukarska, M.,Fianu, I.,Lidschreiber, M.,Naffakh, N.,Dienemann, C.,Cusack, S.,Cramer, P.
Mechanism of co-transcriptional cap snatching by influenza polymerase.
Nature, 2026

PubMed Abstract: Influenza virus mRNAs are stable and competent for nuclear export and translation because they receive a 5' cap(1) structure in a process called cap snatching. During cap snatching, the viral RNA-dependent RNA polymerase (FluPol) binds to host RNA polymerase II (Pol II) and the emerging transcript. The FluPol endonuclease then cleaves a capped RNA fragment that subsequently acts as a primer for the transcription of viral genes. Here we present the cryogenic electron microscopy structure of FluPol bound to a transcribing Pol II in complex with the elongation factor DSIF in the pre-cleavage state. The structure shows that FluPol directly interacts with both Pol II and DSIF, positioning the FluPol endonuclease domain near the RNA exit channel of Pol II. These interactions are important for the endonuclease activity of FluPol and FluPol activity in cells. A second structure, trapped after cap snatching, shows that the cleaved capped RNA rearranges within FluPol, directing the capped RNA 3' end toward the FluPol polymerase active site for viral transcription initiation. Together, our results provide the molecular mechanisms of co-transcriptional cap snatching by FluPol.

PubMed: 41781612
DOI: 10.1038/s41586-026-10189-0

実験手法

ELECTRON MICROSCOPY (3.1 Å)