9FVD

Cryo-EM structure of single-layered nucleoprotein-RNA helical assembly from Marburg virus, trimeric repeat unit

Summary for 9FVD

• Entry DOI: 10.2210/pdb9fvd/pdb
• EMDB information: 50803
• Descriptor: Nucleoprotein, RNA (5'-R(P*AP*GP*AP*CP*AP*CP*AP*CP*AP*AP*AP*AP*AP*CP*AP*AP*GP*A)-3') (2 entities in total)
• Functional Keywords: rna-binding protein, viral protein
• Biological source: Marburg virus - Musoke, Kenya, 1980; More
• Total number of polymer chains: 8
• Total formula weight: 177568.57

Authors

Zinzula, L.,Beck, F.,Camasta, M.,Bohn, S.,Liu, C.,Morado, D.,Bracher, A.,Plitzko, J.M.,Baumeister, W. (deposition date: 2024-06-26, release date: 2024-10-09, Last modification date: 2024-12-25)

Primary citation

Zinzula, L.,Beck, F.,Camasta, M.,Bohn, S.,Liu, C.,Morado, D.,Bracher, A.,Plitzko, J.M.,Baumeister, W.
Cryo-EM structure of single-layered nucleoprotein-RNA complex from Marburg virus.
Nat Commun, 15:10307-10307, 2024

PubMed Abstract: Marburg virus (MARV) causes lethal hemorrhagic fever in humans, posing a threat to global health. We determined by cryogenic electron microscopy (cryo-EM) the MARV helical ribonucleoprotein (RNP) complex structure in single-layered conformation, which differs from the previously reported structure of a double-layered helix. Our findings illuminate novel RNP interactions and expand knowledge on MARV genome packaging and nucleocapsid assembly, both processes representing attractive targets for the development of antiviral therapeutics against MARV disease.

PubMed: 39604358
DOI: 10.1038/s41467-024-54431-7

Experimental method

ELECTRON MICROSCOPY (3.2 Å)