9FTV

Serial microseconds crystallography at ID29 using MPI extruder: Thaumatin

Summary for 9FTV

• Entry DOI: 10.2210/pdb9ftv/pdb
• Descriptor: Thaumatin I, L(+)-TARTARIC ACID (3 entities in total)
• Functional Keywords: serial crystallography, serial microsecond crystallography, room temperature, id29, esrf-ebs, plant protein
• Biological source: Thaumatococcus daniellii
• Total number of polymer chains: 1
• Total formula weight: 22378.13

Authors

Orlans, J.,Rose, S.L.,Basu, S.,de Sanctis, D. (deposition date: 2024-06-25, release date: 2025-01-22)

Primary citation

Orlans, J.,Rose, S.L.,Ferguson, G.,Oscarsson, M.,Homs Puron, A.,Beteva, A.,Debionne, S.,Theveneau, P.,Coquelle, N.,Kieffer, J.,Busca, P.,Sinoir, J.,Armijo, V.,Lopez Marrero, M.,Felisaz, F.,Papp, G.,Gonzalez, H.,Caserotto, H.,Dobias, F.,Gigmes, J.,Lebon, G.,Basu, S.,de Sanctis, D.
Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron.
Commun Chem, 8:6-6, 2025

PubMed Abstract: Serial macromolecular crystallography has become a powerful method to reveal room temperature structures of biological macromolecules and perform time-resolved studies. ID29, a flagship beamline of the ESRF 4th generation synchrotron, is the first synchrotron beamline in the world capable of delivering high brilliance microsecond X-ray pulses at high repetition rate for the structure determination of biological macromolecules at room temperature. The cardinal combination of microsecond exposure times, innovative beam characteristics and adaptable sample environment provides high quality complete data, even from an exceptionally small amount of crystalline material, enabling what we collectively term serial microsecond crystallography (SµX). After validating the use of different sample delivery methods with various model systems, we applied SµX to an integral membrane receptor, where only a few thousands diffraction images were sufficient to obtain a fully interpretable electron density map for the antagonist istradefylline-bound A receptor conformation, providing access to the antagonist binding mode. SµX, as demonstrated at ID29, will quickly find its broad applicability at upcoming 4th generation synchrotron sources worldwide and opens a new frontier in time-resolved SµX.

PubMed: 39775172
DOI: 10.1038/s42004-024-01404-y

Experimental method

X-RAY DIFFRACTION (1.75 Å)