9FTL
Asgard ESCRT-IIIB filament structure
Summary for 9FTL
| Entry DOI | 10.2210/pdb9ftl/pdb |
| EMDB information | 50748 |
| Descriptor | Heimdallarchaeota archaeon AB_125 ESCRT-IIIB (1 entity in total) |
| Functional Keywords | escrt-iii, filament, membrane binding protein, membrane remodelling, asgard archaea, membrane protein |
| Biological source | Candidatus Heimdallarchaeota archaeon |
| Total number of polymer chains | 2 |
| Total formula weight | 48260.54 |
| Authors | |
| Primary citation | Souza, D.P.,Espadas, J.,Chaaban, S.,Moody, E.R.R.,Hatano, T.,Balasubramanian, M.,Williams, T.A.,Roux, A.,Baum, B. Asgard archaea reveal the conserved principles of ESCRT-III membrane remodeling. Sci Adv, 11:eads5255-eads5255, 2025 Cited by PubMed Abstract: ESCRT-III proteins assemble into composite polymers that undergo stepwise changes in composition and structure to deform membranes across the tree of life. Here, using a phylogenetic analysis, we demonstrate that the two endosomal sorting complex required for transport III (ESCRT-III) proteins present in eukaryote's closest Asgard archaeal relatives are evolutionarily related to the B- and A-type eukaryotic paralogs that initiate and execute membrane remodeling, respectively. We show that Asgard ESCRT-IIIB assembles into parallel arrays on planar membranes to initiate membrane deformation, from where it recruits ESCRT-IIIA to generate composite polymers. Last, we show that Asgard ESCRT-IIIA is able to remodel membranes into tubes as a likely prelude to scission. Together, these data reveal a set of conserved principles governing ESCRT-III-dependent membrane remodeling that first emerged in a two-component ESCRT-III system in archaea. PubMed: 39919172DOI: 10.1126/sciadv.ads5255 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.91 Å) |
Structure validation
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