9FO1

Family S1_16 sulfatase from metagenomic dataset AMOR

Summary for 9FO1

• Entry DOI: 10.2210/pdb9fo1/pdb
• Descriptor: k-carrageenan sulfatase S1_16, CALCIUM ION, GLYCEROL, ... (4 entities in total)
• Functional Keywords: carrageenan sulfatase, 4s-galactose specific sulfatase, marine metagenome, hydrolase
• Biological source: Bryobacteraceae
• Total number of polymer chains: 1
• Total formula weight: 53141.33

Authors

Rhein-Knudsen, N.,Horn, S.J.,Jeudy, A.,Czjzek, M. (deposition date: 2024-06-11, release date: 2025-01-22, Last modification date: 2025-02-05)

Primary citation

Rhein-Knudsen, N.,Reyes-Weiss, D.S.,Klau, L.J.,Jeudy, A.,Roret, T.,Stokke, R.,Eijsink, V.G.H.,Aachmann, F.L.,Czjzek, M.,Horn, S.J.
Identification and Characterization of a New Thermophilic kappa-Carrageenan Sulfatase.
J.Agric.Food Chem., 73:2044-2055, 2025

PubMed Abstract: Carrageenans are sulfated polysaccharides found in the cell wall of certain red seaweeds. They are widely used in the food industry for their gelling and stabilizing properties. In nature, carrageenans undergo enzymatic modification and degradation by marine organisms. Characterizing these enzymes is crucial for understanding carrageenan utilization and may eventually enable the development of targeted processes to modify carrageenans for industrial applications. In our study, we characterized a κ-carrageenan sulfatase, AMOR_S1_16A, belonging to the sulfatase S1_16 subfamily, which selectively desulfates the nonreducing end galactoses of κ-carrageenan oligomers in an exomode. Notably, AMOR_S1_16A represents the first κ-carrageenan sulfatase within the S1_16 subfamily and exhibits a novel enzymatic activity. This study provides further understanding of the substrate specificity and characteristics of the S1_16 subfamily. Moreover, this research highlights that many processes and enzymes remain to be discovered to fully understand carrageenan utilization pathways and to develop enzymatic processes for carrageenan modification and processing.

PubMed: 39797788
DOI: 10.1021/acs.jafc.4c09751

Experimental method

X-RAY DIFFRACTION (3.11 Å)