9FML

Aerolysin heptamer in membrane inserted form reconstituted in amphipoles.

これはPDB形式変換不可エントリーです。

9FML の概要

• エントリーDOI: 10.2210/pdb9fml/pdb
• EMDBエントリー: 50562
• 分子名称: Aerolysin (1 entity in total)
• 機能のキーワード: pore forming toxin, aerolysin, amphipole reconstitution, cryo-em, toxin
• 由来する生物種: Aeromonas hydrophila
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 363863.17

構造登録者

Iacovache, I.,Zuber, B. (登録日: 2024-06-06, 公開日: 2025-02-12, 最終更新日: 2025-02-19)

主引用文献

Anton, J.S.,Iacovache, I.,Bada Juarez, J.F.,Abriata, L.A.,Perrin, L.W.,Cao, C.,Marcaida, M.J.,Zuber, B.,Dal Peraro, M.
Aerolysin Nanopore Structures Revealed at High Resolution in a Lipid Environment.
J.Am.Chem.Soc., 147:4984-4992, 2025

PubMed Abstract: Aerolysin is a β-pore-forming toxin produced by most Aeromonas bacteria, which has attracted large attention in the field of nanopore sensing due to its narrow and charged pore lumen. Structurally similar proteins, belonging to the aerolysin-like family, are present throughout all kingdoms of life, but very few of them have been structurally characterized in a lipid environment. Here, we present the first high-resolution atomic cryo-EM structures of aerolysin prepore and pore in a membrane-like environment. These structures allow the identification of key interactions, which are relevant for understanding the pore formation mechanism and for correctly positioning the pore β-barrel and its anchoring β-turn motif in the membrane. Moreover, we elucidate at high resolution the architecture of key pore mutations and precisely identify four constriction rings in the pore lumen that are highly relevant for nanopore sensing experiments.

PubMed: 39900531
DOI: 10.1021/jacs.4c14288

実験手法

ELECTRON MICROSCOPY (2.2 Å)