Summary for 9F58
| Entry DOI | 10.2210/pdb9f58/pdb |
| EMDB information | 50188 |
| Descriptor | eIF-2-alpha kinase GCN2, 60S ribosomal protein L9-A, 60S ribosomal protein L10, ... (46 entities in total) |
| Functional Keywords | lsu, 60s, gcn2, ribosome |
| Biological source | Saccharomyces cerevisiae (brewer's yeast) More |
| Total number of polymer chains | 50 |
| Total formula weight | 3073636.65 |
| Authors | Paternoga, H.,Dimitrova-Paternoga, L.,Wilson, D.N. (deposition date: 2024-04-28, release date: 2025-03-26, Last modification date: 2025-05-21) |
| Primary citation | Paternoga, H.,Xia, L.,Dimitrova-Paternoga, L.,Li, S.,Yan, L.L.,Oestereich, M.,Kasvandik, S.,Nanjaraj Urs, A.N.,Beckert, B.,Tenson, T.,Zaher, H.,Inada, T.,Wilson, D.N. Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit. Proc.Natl.Acad.Sci.USA, 122:e2415807122-e2415807122, 2025 Cited by PubMed Abstract: The integrated stress response (ISR) is a central signaling network that enables eukaryotic cells to respond to a variety of different environmental stresses. Such stresses cause ribosome collisions that lead to activation of the kinase Gcn2, resulting in the phosphorylation and inactivation of eukaryotic initiation factor 2 and thereby promoting selective translation of mRNAs to restore homeostasis. Despite the importance of the ISR and intensive study over the past decades, structural insight into how Gcn2 interacts with ribosomal particles has been lacking. Using ex vivo affinity purification approaches, we have obtained a cryoelectron microscopy structure of a yeast Gcn2 dimer in complex with the ribosomal 60S subunit. The Gcn2 dimer is formed by dimerization of the histidine tRNA synthetase-like domains, which establish extensive interactions with the stalk-base and sarcin-ricin loop of the 60S subunit. The C-terminal domain of Gcn2 is also dimerized and occupies the A- and P-site tRNA binding sites at the peptidyl-transferase center of the 60S subunit. Complementary functional studies indicate that binding of Gcn2 to the 60S subunit does not require the coactivators Gcn1 or Gcn20, nor does it lead to phosphorylation of eIF2α. Instead, upon stress, we observe a shift of Gcn2 from the 60S subunit into the colliding ribosome fraction, suggesting that the Gcn2-60S complex represents an inactive stand-by state to enable a rapid redistribution to collided ribosomes, and thereby facilitating a quick and efficient response to stress. PubMed: 40198700DOI: 10.1073/pnas.2415807122 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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