9EWC

DNA Polymerase Lambda I493R 528-530 NEY, TTP:At Ca2+ Ground State Ternary Complex

9EWC の概要

• エントリーDOI: 10.2210/pdb9ewc/pdb
• 関連するPDBエントリー: 9ewb
• 分子名称: DNA polymerase lambda, DNA template strand, DNA primer strand upstream, ... (8 entities in total)
• 機能のキーワード: dna polymerase gap filling, nuclear protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 86603.84

構造登録者

Nourisson, A.,Haouz, A.,Missoury, S.,Delarue, M. (登録日: 2024-04-03, 公開日: 2025-07-23, 最終更新日: 2025-09-10)

主引用文献

Nourisson, A.,Missoury, S.,Lucas-Staat, S.,Haouz, A.,Delarue, M.
Fidelity, specialization, and evolution of Paramecium PolX DNA polymerases involved in programmed double-strand break DNA repair.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Repairing programmed DNA double-strand breaks (DSBs) is crucial in the lifecycle of Paramecium tetraurelia, especially during its sexual reproduction phase when its somatic polyploid macronucleus is lost. The formation of a new macronucleus involves programmed genome rearrangements, introducing DNA DSBs at ∼45 000 loci. Paramecium tetraurelia employs a non-homologous end joining (NHEJ) mechanism for the faithful repair of these DSBs. There are four genes encoding DNA polymerases of family X in the genome, one of which was found recently to colocalize with other NHEJ proteins in the nucleus. Here we have characterized all four enzymes and shown that they are generally very faithful. They fall into two functional classes that may specialize in the distinct repair contexts encountered during DSB DNA repair. Biochemical assays, site-directed mutagenesis, and X-ray structures of mutants of human Polλ incorporating sequence determinants from P. tetraurelia PolX or metazoan Polβ are used to investigate the origin of their fidelity. Our findings suggest that Paramecium PolX enzymes may represent evolutionary intermediates between metazoan Polβ and Polλ. A general classification of DNA PolXs based on clustering methods indicates that our results can be generalized to plant DNA PolXs (Polλ-like) involved in DSB DNA repair generated by CRISPR-Cas9 engineering.

PubMed: 40829804
DOI: 10.1093/nar/gkaf786

実験手法

X-RAY DIFFRACTION (3.67 Å)