9ETT
Structure of the archaellum of Sulfolobus acidocaldarius strain MW039 (delta agl3 mutant).
This is a non-PDB format compatible entry.
Summary for 9ETT
| Entry DOI | 10.2210/pdb9ett/pdb |
| Related | 8QX4 |
| EMDB information | 19961 |
| Descriptor | Flagellin, alpha-D-mannopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total) |
| Functional Keywords | cell surface appendage, n-glycosylation, mutagenesis, protein fibril |
| Biological source | Sulfolobus acidocaldarius |
| Total number of polymer chains | 20 |
| Total formula weight | 694570.20 |
| Authors | Daum, B.,Isupov, M.N.,Gaines, M.,McLaren, M.,Mollat, C. (deposition date: 2024-03-26, release date: 2025-02-05) |
| Primary citation | Gaines, M.C.,Isupov, M.N.,McLaren, M.,Mollat, C.L.,Haque, R.U.,Stephenson, J.K.,Sivabalasarma, S.,Hanus, C.,Kattnig, D.,Gold, V.A.M.,Albers, S.,Daum, B. Towards a molecular picture of the archaeal cell surface. Nat Commun, 15:10401-10401, 2024 Cited by PubMed Abstract: Archaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These include rotary swimming propellers analogous to bacterial flagella (archaella), pili for twitching motility (Aap), adhesive fibres (threads), and filaments facilitating homologous recombination upon UV stress (UV pili). Here, we use cryo-electron microscopy to describe the structure of the S. acidocaldarius archaellum at 2.0 Å resolution, and update the structures of the thread and the Aap pilus at 2.7 Å and 2.6 Å resolution, respectively. We define features unique to archaella of the order Sulfolobales and compare their structure to those of Aap and threads in the context of the S-layer. We define distinct N-glycan patterns in the three filaments and identify a putative O-glycosylation site in the thread. Finally, we ascertain whether N-glycan truncation leads to structural changes in archaella and Aap. PubMed: 39614099DOI: 10.1038/s41467-024-53986-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.37 Å) |
Structure validation
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