9EOQ

Cryo-EM Structure of a 1033 Scaffold Base DNA Origami Nanostructure V4 and TBA

Summary for 9EOQ

• Entry DOI: 10.2210/pdb9eoq/pdb
• EMDB information: 19867
• Descriptor: DNA (42-MER), DNA (5'-D(P*AP*TP*AP*TP*AP*GP*CP*GP*TP*GP*GP*AP*AP*GP*T)-3') (2 entities in total)
• Functional Keywords: dna origami, multilayer, square lattice, 1033 scaffold, dna, tba
• Biological source: synthetic construct; More
• Total number of polymer chains: 2
• Total formula weight: 17707.39

Authors

Ali, K.,Georg, K.,Volodymyr, M.,Johanna, G.,Maximilian, N.H.,Lukas, K.,Simone, C.,Hendrik, D. (deposition date: 2024-03-15, release date: 2024-04-24, Last modification date: 2024-05-08)

Primary citation

Khoshouei, A.,Kempf, G.,Mykhailiuk, V.,Griessing, J.M.,Honemann, M.N.,Kater, L.,Cavadini, S.,Dietz, H.
Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM.
Nano Lett., 24:5031-5038, 2024

PubMed Abstract: DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.

PubMed: 38602296
DOI: 10.1021/acs.nanolett.4c00915

Experimental method

ELECTRON MICROSCOPY (7.5 Å)