9ED9

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State III - checkpoint)

Summary for 9ED9

• Entry DOI: 10.2210/pdb9ed9/pdb
• Related: 9EAK 9EAL
• EMDB information: 47941
• Descriptor: sgRNA, PAM-distal Target Strand, Non-target strand, ... (5 entities in total)
• Functional Keywords: crispr-associated endonuclease, dna binding protein, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 5
• Total formula weight: 223883.28

Authors

Kiernan, K.A.,Taylor, D.W. (deposition date: 2024-11-16, release date: 2025-06-18)

• Primary citation: Kiernan, K.A.,Taylor, D.W.; Kinetics-guided cryo-EM enables visualization of Cas9 dissociation post-cleavage; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.95 Å)