9E9F

Pseudomonas aeruginosa MexY

9E9F の概要

• エントリーDOI: 10.2210/pdb9e9f/pdb
• EMDBエントリー: 47796
• 分子名称: Efflux pump membrane transporter (1 entity in total)
• 機能のキーワード: pseudomonsa aeruginosa, mexy, rnd, efflux, membrane protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 333863.74

構造登録者

Gregor, W.D.,Maharjan, R.,Zhang, Z.,Yu, E.W. (登録日: 2024-11-08, 公開日: 2024-12-18, 最終更新日: 2026-01-21)

主引用文献

Gregor, W.D.,Maharjan, R.,Zhang, Z.,Chiaraviglio, L.,Sastry, N.,Cui, M.,Kirby, J.E.,Yu, E.W.
Cryo-EM structure of the Pseudomonas aeruginosa MexY multidrug efflux pump.
Mbio, 16:e0382624-e0382624, 2025

PubMed Abstract: , a Gram-negative pathogen, has emerged as one of the most highly antibiotic-resistant bacteria worldwide and subsequently has become a leading cause of healthcare-associated, life-threatening infections. multidrug efflux Y (MexY) is an efflux pump that belongs to the resistance-nodulation-cell division (RND) superfamily. It is a major determinant for resistance to aminoglycosides in this opportunistic pathogen. However, the detailed molecular mechanisms involved in aminoglycoside recognition and extrusion by MexY have not been elucidated. Here, we report the cryo-electron microscopy structure of MexY to a resolution of 3.63 Å. The structure directly indicates two plausible pathways for drug export. It also suggests that MexY is capable of picking up antibiotics via the ceiling of the central cavity formed by the MexY trimer. Molecular dynamics simulations depict that MexY is able to use a tunnel connecting the central cavity to the funnel of the trimer to export its substrates.

PubMed: 40042268
DOI: 10.1128/mbio.03826-24

実験手法

ELECTRON MICROSCOPY (3.63 Å)