9E7M
In situ cryoEM structure of bacteriophage Ur-lambda tail tip complex
This is a non-PDB format compatible entry.
Summary for 9E7M
| Entry DOI | 10.2210/pdb9e7m/pdb |
| EMDB information | 47685 |
| Descriptor | Tape measure protein, Tail tip assembly protein I, Tip attachment protein J, ... (9 entities in total) |
| Functional Keywords | bacteriophage, tail tip complex, viral protein |
| Biological source | Escherichia phage Lambda More |
| Total number of polymer chains | 36 |
| Total formula weight | 689336.21 |
| Authors | Yu, H.,Liu, J.,Molineux, I.J. (deposition date: 2024-11-03, release date: 2025-11-05, Last modification date: 2025-11-26) |
| Primary citation | Yu, H.,Wang, C.,Yue, J.,Guo, W.,Molineux, I.J.,Liu, J. Structural basis of bacteriophage Ur-lambda infection initiation. Sci Adv, 11:eadw7914-eadw7914, 2025 Cited by PubMed Abstract: Bacteriophages must recognize host receptors and penetrate the host cell envelope to initiate infection. How the classic phage λ initiates infection is not yet understood. Here, we combine cryo-electron microscopy and tomography to visualize infection initiation by Ur-λ, the original λ isolate that uses side fibers to adsorb rapidly to . We determine the structure of Ur-λ, resolving the full-length central and side fibers, thus providing a structural basis for host recognition. We show that Ur-λ contains six copies of its tape measure protein. We capture intermediates of the tail tip complex during infection initiation, revealing how extensive conformational changes enable adsorption, and visualize the trans-envelope channel required for genome ejection. PubMed: 41237242DOI: 10.1126/sciadv.adw7914 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.37 Å) |
Structure validation
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