9E6L

Cryo-EM structure of yeast Rad51 nucleoprotein filament bound to Rad54peptide

Summary for 9E6L

• Entry DOI: 10.2210/pdb9e6l/pdb
• EMDB information: 47572
• Descriptor: DNA repair and recombination protein RAD54, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA repair protein RAD51, ... (5 entities in total)
• Functional Keywords: rad51, dna recombinase, homologous recombination, dna repair, recombination, recombination-dna complex, recombination/dna
• Biological source: Saccharomyces cerevisiae (brewer's yeast); More
• Total number of polymer chains: 12
• Total formula weight: 238514.33

Authors

Shin, Y.,Greene, E.C. (deposition date: 2024-10-30, release date: 2025-01-22, Last modification date: 2025-10-01)

Primary citation

Shin, Y.,Petassi, M.T.,Jessop, A.M.,Kim, S.Y.,Matei, R.,Morse, K.,Raina, V.B.,Roy, U.,Greene, E.C.
Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination.
Proc.Natl.Acad.Sci.USA, 122:e2510007122-e2510007122, 2025

PubMed Abstract: Rad51 catalyzes the DNA pairing reactions that take place during homologous recombination (HR), and HR must be tightly regulated to ensure physiologically appropriate outcomes. Rad54 is an ATP-dependent DNA motor protein that stimulates Rad51 activity during mitosis. In meiosis Rad51 is downregulated by the protein Hed1, which blocks Rad54 binding to Rad51, and allows Dmc1 to function as the active recombinase. We currently have a poor understanding of the regulatory interplay between Rad54, Hed1, Rad51, and Dmc1. Here, we identify a conserved Rad51 interaction motif within Rad54, and we solve a CryoEM structure of this motif bound to Rad51. We also identify a distinct Rad51 interaction motif within Hed1 and solve its structure bound to Rad51. These structures explain how Rad54 engages Rad51 to promote recombination between sister chromatids during mitosis and how Rad51 is downregulated by Hed1 upon entry into meiosis such that its meiosis-specific homolog Dmc1 can promote recombination between homologous chromosomes.

PubMed: 40932772
DOI: 10.1073/pnas.2510007122

Experimental method

ELECTRON MICROSCOPY (3.3 Å)