9D8D
Crystal structure of Vibrio cholerae NFeoB in the GMPPCP-bound form
Summary for 9D8D
| Entry DOI | 10.2210/pdb9d8d/pdb |
| Descriptor | Ferrous iron transport protein B, PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, GLYCEROL, ... (5 entities in total) |
| Functional Keywords | feo, iron, transport, transport protein |
| Biological source | Vibrio cholerae |
| Total number of polymer chains | 4 |
| Total formula weight | 119623.89 |
| Authors | Lee, M.,Magante, K.D.,Smith, A.T. (deposition date: 2024-08-19, release date: 2025-09-03, Last modification date: 2026-03-18) |
| Primary citation | Magante, K.,Armstrong, C.M.,Lee, M.,Smith, A.T. The Structure of the Full Catalytic Cycle of Vibrio cholerae NFeoB. J.Mol.Biol., 438:169727-169727, 2026 Cited by PubMed Abstract: The acquisition of iron is critical for the survival and the virulence of numerous infectious pathogens, and most bacteria acquire ferrous iron (Fe) by utilizing the ferrous iron transport (Feo) system. FeoB is the main component of this system and its function is regulated by its soluble cytosolic domain, termed NFeoB. We have recently begun to define the structure and the mechanism of the Feo system from the bacterium Vibrio cholerae, the causative agent of the disease cholera. However, major structural gaps in our understanding of the nucleotide-promiscuous V. cholerae NFeoB still exist. In this work, we have determined several new X-ray crystal structures that reveal distinct snapshots of the VcNFeoB domain in uncommon and unprecedented states, ultimately illuminating the full catalytic cycle of this NTPase. This work reveals important functional features of VcNFeoB that may be leveraged and ultimately targeted to prevent the infectivity and the spread of cholera. PubMed: 41724262DOI: 10.1016/j.jmb.2026.169727 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.11 Å) |
Structure validation
Download full validation report
