9CIW

Penguinpox cGAMP PDE H72A mutant in complex with 2'3'-cGAMP

This is a non-PDB format compatible entry.

Summary for 9CIW

• Entry DOI: 10.2210/pdb9ciw/pdb
• Descriptor: Penguinpox cGAMP PDE, [(2~{R},3~{R},4~{R},5~{S})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl [(2~{R},3~{R},4~{S},5~{R})-2-(2-azanyl-6-oxidanylidene-1~{H}-purin-9-yl)-5-(hydroxymethyl)-4-oxidanyl-oxolan-3-yl] hydrogen phosphate (3 entities in total)
• Functional Keywords: immune evasion, phosphodiesterase, viral protein
• Biological source: Penguinpox virus
• Total number of polymer chains: 4
• Total formula weight: 92689.73

Authors

Hobbs, S.J.,Nomburg, J.,Doudna, J.A.,Kranzusch, P.J. (deposition date: 2024-07-05, release date: 2024-09-04, Last modification date: 2024-10-16)

Primary citation

Hobbs, S.J.,Nomburg, J.,Doudna, J.A.,Kranzusch, P.J.
Animal and bacterial viruses share conserved mechanisms of immune evasion.
Cell, 187:5530-5539.e8, 2024

PubMed Abstract: Animal and bacterial cells sense and defend against viral infections using evolutionarily conserved antiviral signaling pathways. Here, we show that viruses overcome host signaling using mechanisms of immune evasion that are directly shared across the eukaryotic and prokaryotic kingdoms of life. Structures of animal poxvirus proteins that inhibit host cGAS-STING signaling demonstrate architectural and catalytic active-site homology shared with bacteriophage Acb1 proteins, which inactivate CBASS anti-phage defense. In bacteria, phage Acb1 proteins are viral enzymes that degrade host cyclic nucleotide immune signals. Structural comparisons of poxvirus protein-2'3'-cGAMP and phage Acb1-3'3'-cGAMP complexes reveal a universal mechanism of host nucleotide immune signal degradation and explain kingdom-specific additions that enable viral adaptation. Chimeric bacteriophages confirm that animal poxvirus proteins are sufficient to evade immune signaling in bacteria. Our findings identify a mechanism of immune evasion conserved between animal and bacterial viruses and define shared rules that explain host-virus interactions across multiple kingdoms of life.

PubMed: 39197447
DOI: 10.1016/j.cell.2024.07.057

Experimental method

X-RAY DIFFRACTION (2.4 Å)