9C9L

Cryo-EM structure of the C1q A, B-crt, C peptide assembly narrow region

Summary for 9C9L

• Entry DOI: 10.2210/pdb9c9l/pdb
• EMDB information: 45363
• Descriptor: Peptide from Complement C1q subcomponent subunit B, Peptide from Complement C1q subcomponent subunit A, Peptide from Complement C1q subcomponent subunit C (3 entities in total)
• Functional Keywords: c1q, complement, collagen, triple helix, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 18
• Total formula weight: 22933.59

Authors

Kreutzberger, M.A.,Yu, L.T.,Egelman, E.H.,Hartgerink, J.D. (deposition date: 2024-06-14, release date: 2025-02-12, Last modification date: 2025-03-19)

Primary citation

Kreutzberger, M.A.B.,Yu, L.T.,Bui, T.H.,Hancu, M.C.,Purdy, M.D.,Osinski, T.,Kasson, P.M.,Egelman, E.H.,Hartgerink, J.D.
A Collagen Triple Helix without the Superhelical Twist.
Acs Cent.Sci., 11:331-345, 2025

PubMed Abstract: Collagens are ubiquitous in biology: functioning as the backbone of the extracellular matrix, forming the primary structural components of key immune system complexes, and fulfilling numerous other structural roles in a variety of systems. Despite this, there is limited understanding of how triple helices, the basic collagen structural units, pack into collagenous assemblies. Here we use a peptide self-assembly system to design collagenous assemblies based on the C1q collagen-like region. Using cryo-EM we solved a structure of one assembly to 3.5 Å resolution and built an atomic model. From this, we identify a triple helix conformation with no superhelical twist, starkly in contrast to the canonical right-handed triple helix. This nontwisting region allows for unique hydroxyproline stacking between adjacent triple helices and also results in the formation of an exposed cavity with rings of hydrophobic amino acids packed symmetrically. We find no precedent for such an arrangement of collagen triple helices and designed assemblies with substituted amino acids in various locations to probe key stabilizing amino acid interactions in the complex. The stability of these altered complexes behaves as predicted by our atomic model. Our findings, combined with the extremely limited experimental structural data on triple helix packing in the literature, suggest that collagen and collagen-like assemblies may adopt a far more varied conformational landscape than previously appreciated. We hypothesize that this is particularly likely in packed assemblies of triple helices, adjacent to the termini of these helices and at discontinuities in the required Xaa-Yaa-Gly repeating primary sequence, a discontinuity found in the majority of this class of proteins and in many collagen-associated diseases.

PubMed: 40028357
DOI: 10.1021/acscentsci.5c00018

Experimental method

ELECTRON MICROSCOPY (3.7 Å)