9C4Q
Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with newly designed herbicide FMO
This is a non-PDB format compatible entry.
Summary for 9C4Q
| Entry DOI | 10.2210/pdb9c4q/pdb |
| Related | 5k2o |
| Descriptor | Acetolactate synthase, chloroplastic, FORMIC ACID, SULFATE ION, ... (12 entities in total) |
| Functional Keywords | herbicide, resistance, ahas, als, ligase, transferase |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 1 |
| Total formula weight | 67044.47 |
| Authors | |
| Primary citation | Cheng, Y.,Wang, Y.,Lonhienne, T.,Wang, J.G.,Guddat, L.W. Crystal Structures of Arabidopsis thaliana Acetohydroxyacid Synthase in Complex with the Herbicide Triasulfuron and Two Analogues with Herbicidal Activity in Field Trials. J.Agric.Food Chem., 2024 Cited by PubMed Abstract: Triasulfuron is a commercial herbicide of the sulfonylurea family. This compound targets acetohydroxyacid synthase (AHAS, E.C. 2.2.1.6), the first enzyme in the branched chain amino acid biosynthesis pathway. Here, we have determined crystal structures of AHAS (AHAS) in complex with triasulfuron and two newly designed herbicidal compounds, identified as FMO and CMO, showing that their binding modes are subtly different. Kinetic studies showed all three compounds exhibit varying values, 0.192 ± 0.013 μM for triasulfuron, 0.086 ± 0.013 μM for FMO, and 1.448 ± 0.058 μM for CMO, but all are strong time-dependent accumulative inhibitors of AHAS. Apart from triasulfuron being a powerful herbicide with application rates of 10-15 g/ha in wheat fields, CMO and FMO are also herbicidal at 7.5-30 g/ha for barnyard grass. This study emphasizes that accumulative inhibition is an important factor that contributes to herbicidal activity. PubMed: 39373624DOI: 10.1021/acs.jafc.4c04990 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.52 Å) |
Structure validation
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