9C42

Structure of human MR1-ellagic acid in complex with human MAIT A-F7 TCR

Summary for 9C42

• Entry DOI: 10.2210/pdb9c42/pdb
• Descriptor: Major histocompatibility complex class I-related gene protein, Beta-2-microglobulin, TRA@ protein, ... (10 entities in total)
• Functional Keywords: t-cell receptor, mhc-like, inhibitor, antigen-presenting molecule, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 189265.35

Authors

Wang, C.J.H.,Le Nours, J.,Rossjohn, J. (deposition date: 2024-06-02, release date: 2025-06-11, Last modification date: 2026-03-11)

Primary citation

O'Sullivan, D.,Wang, C.J.H.,Tang, J.S.,Stephens, R.,Faulkner, S.,Gell, K.,Li, Y.,Cait, A.,McInerney, M.,Purcell, A.W.,Rossjohn, J.,Le Nours, J.,Gasser, O.
Ellagic acid metabolism as a source of dietary MR1 ligands.
J Immunol., 215:-, 2026

PubMed Abstract: MR1 is an major histocompatibility complex class I-like molecule that presents small molecule metabolites to MR1-restricted T cells that include a major population of highly conserved T cells known as mucosal-associated invariant T (MAIT) cells. MAIT cells recognize bacterial riboflavin pathway-derived neoantigens and are being attributed an increasing number of immune and homeostatic functions. However, the chemical breadth and diversity of MR1-restricted ligands remain to be fully elucidated. Due to the largely (poly)cyclic structure of known MR1 ligands, we aimed to identify MR1 ligands from a library of dietary phenolic metabolites. Competitive MAIT cell inhibition assays using both cell lines and primary cells isolated from human blood identified gut microbial metabolites of ellagitannins that include ellagic acid (EA), urolithin D (UroD), and UroM5 as potential MR1 ligands. Fluorescence polarization binding assays demonstrated that EA, UroM5, UroC, and UroB bound to MR1, and we provide a structural basis for EA presentation by MR1. Overall, our findings indicate that EA metabolism provides dietary MR1 ligands that inhibit T cell receptor-dependent MAIT cell activation.

PubMed: 41758608
DOI: 10.1093/jimmun/vkaf346

Experimental method

X-RAY DIFFRACTION (2.69 Å)