9C0I
Structure of the DRT2 reverse transcriptase in complex with its non-coding RNA
Summary for 9C0I
| Entry DOI | 10.2210/pdb9c0i/pdb |
| EMDB information | 45085 |
| Descriptor | Reverse transcriptase/maturase family protein, DNA primer, DRT2 ncRNA, ... (5 entities in total) |
| Functional Keywords | reverse transcriptase, rolling circle amplification, phage defense, transferase-dna-rna complex, transferase/dna/rna |
| Biological source | Klebsiella pneumoniae More |
| Total number of polymer chains | 3 |
| Total formula weight | 142322.53 |
| Authors | Wilkinson, M.E.,Zhang, F. (deposition date: 2024-05-25, release date: 2024-09-04, Last modification date: 2024-10-16) |
| Primary citation | Wilkinson, M.E.,Li, D.,Gao, A.,Macrae, R.K.,Zhang, F. Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA. Science, 386:eadq3977-eadq3977, 2024 Cited by PubMed Abstract: Reverse transcription has frequently been co-opted for cellular functions and in prokaryotes is associated with protection against viral infection, but the underlying mechanisms of defense are generally unknown. Here, we show that in the DRT2 defense system the reverse transcriptase binds a neighboring pseudoknotted noncoding RNA. Upon bacteriophage infection, a template region of this RNA is reverse transcribed into an array of tandem repeats that reconstitute a promoter and open reading frame, allowing expression of a toxic repetitive protein and an abortive infection response. Biochemical reconstitution of this activity and cryogenic electron microscopy provide a molecular basis for repeat synthesis. Gene synthesis from a noncoding RNA is a new mode of genetic regulation in prokaryotes. PubMed: 39208082DOI: 10.1126/science.adq3977 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.91 Å) |
Structure validation
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