9BW4

TXNL1-bound proteasome

Summary for 9BW4

• Entry DOI: 10.2210/pdb9bw4/pdb
• EMDB information: 44952
• Descriptor: 26S proteasome non-ATPase regulatory subunit 1, 26S proteasome non-ATPase regulatory subunit 8, 26S proteasome complex subunit SEM1, ... (37 entities in total)
• Functional Keywords: proteasome, nuclear protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 40
• Total formula weight: 1503010.57

Authors

Gao, J.,Yip, M.C.J.,Shao, S. (deposition date: 2024-05-20, release date: 2025-06-11, Last modification date: 2025-12-24)

Primary citation

Gao, J.,Nardone, C.,Yip, M.C.J.,Chino, H.,Gu, X.,Mirman, Z.,Rale, M.J.,Paulo, J.A.,Elledge, S.J.,Shao, S.
Structure of the TXNL1-bound proteasome.
Nat.Struct.Mol.Biol., 32:2398-2402, 2025

PubMed Abstract: Proteasomes degrade diverse proteins in different cellular contexts through incompletely defined regulatory mechanisms. Here we report the cryo-EM structure of human thioredoxin-like protein 1 (TXNL1) bound to the 19S regulatory particle of proteasomes via interactions with PSMD1 (Rpn2), PSMD4 (Rpn10) and PSMD14 (Rpn11). Proteasome binding is necessary for the ubiquitin-independent degradation of TXNL1 upon cellular exposure to metal- or metalloid-containing oxidative agents, thereby establishing a structural requirement for the stress-induced degradation of TXNL1.

PubMed: 40770113
DOI: 10.1038/s41594-025-01639-w

Experimental method

ELECTRON MICROSCOPY (3.3 Å)