9BU6

Vaccine elicited Fab C968.180 with influenza H10 JD13 HA trimer

Summary for 9BU6

• Entry DOI: 10.2210/pdb9bu6/pdb
• EMDB information: 44901
• Descriptor: Fab C968.180 heavy chain, Fab C968.180 light chain, Hemagglutinin HA1, ... (7 entities in total)
• Functional Keywords: vaccine, viral protein-immune system complex, immune system, viral protein/immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 12
• Total formula weight: 269927.82

Authors

Gorman, J.,Kwong, P.D. (deposition date: 2024-05-16, release date: 2024-11-27, Last modification date: 2025-01-22)

Primary citation

Mantus, G.E.,Chopde, A.J.,Gorman, J.,Cominsky, L.Y.,Ourahmane, A.,Creanga, A.,Shimberg, G.D.,Gillespie, R.A.,Van Wazer, D.J.,Zhou, T.,Gajjala, S.R.,Williams, C.,Maestle, E.,Reed, D.S.,Serebryannyy, L.,Costner, P.,Holman, L.,Casazza, J.P.,Koup, R.A.,Dropulic, L.K.,Kwong, P.D.,McDermott, A.B.,Kanekiyo, M.,Andrews, S.F.
Vaccination with different group 2 influenza subtypes alters epitope targeting and breadth of hemagglutinin stem-specific human B cells.
Sci Transl Med, 17:eadr8373-eadr8373, 2025

PubMed Abstract: The conserved influenza hemagglutinin stem, which is a target of cross-neutralizing antibodies, is now used in vaccine strategies focused on protecting against influenza pandemics. Antibody responses to group 1 stem have been extensively characterized, but little is known about group 2. Here, we characterized the stem-specific repertoire of individuals vaccinated with one of three group 2 influenza subtypes (H3, H7, or H10). Epitope mapping revealed two epitope supersites on the group 2 stem. Antibodies targeting the central epitope were broadly cross-reactive, whereas antibodies targeting the lower epitope had narrower breadth but higher potency against H3 subtypes. The ratio of B cells targeting each of the supersites varied with the vaccine subtype, leading to differences in the cross-reactivity of the B cell response. Our findings suggest that vaccine strategies targeting both group 2 stem epitopes would be complementary, eliciting broader and more potent protection against both seasonal and pandemic influenza strains.

PubMed: 39742506
DOI: 10.1126/scitranslmed.adr8373

Experimental method

ELECTRON MICROSCOPY (3.65 Å)