9BIO
Structure of VRC44.01 Fab in complex with 3BNC117-purified C1080.c3 RnS SOSIP.664 HIV-1 Env trimer
This is a non-PDB format compatible entry.
Summary for 9BIO
| Entry DOI | 10.2210/pdb9bio/pdb |
| EMDB information | 44595 |
| Descriptor | Envelope glycoprotein gp41, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (14 entities in total) |
| Functional Keywords | hiv-1, sosip, vaccine, therapeutic, viral protein-immune system complex, subunit, multi-donor, viral protein/immune system |
| Biological source | Human immunodeficiency virus 1 (HIV-1) More |
| Total number of polymer chains | 18 |
| Total formula weight | 477437.03 |
| Authors | Gorman, J.,Kwong, P.D. (deposition date: 2024-04-23, release date: 2024-11-13, Last modification date: 2025-01-01) |
| Primary citation | Cale, E.M.,Shen, C.H.,Olia, A.S.,Radakovich, N.A.,Rawi, R.,Yang, Y.,Ambrozak, D.R.,Bennici, A.K.,Chuang, G.Y.,Crooks, E.D.,Driscoll, J.I.,Lin, B.C.,Louder, M.K.,Madden, P.J.,Messina, M.A.,Osawa, K.,Stewart-Jones, G.B.E.,Verardi, R.,Vrakas, Z.,Xie, D.,Zhang, B.,Binley, J.M.,Connors, M.,Koup, R.A.,Pierson, T.C.,Doria-Rose, N.A.,Kwong, P.D.,Mascola, J.R.,Gorman, J. A multidonor class of highly glycan-dependent HIV-1 gp120-gp41 interface-targeting broadly neutralizing antibodies. Cell Rep, 43:115010-115010, 2024 Cited by PubMed Abstract: Antibodies that target the gp120-gp41 interface of the HIV-1 envelope (Env) trimer comprise a commonly elicited category of broadly neutralizing antibodies (bNAbs). Here, we isolate and characterize VRC44, a bNAb lineage with up to 52% neutralization breadth. The cryoelectron microscopy (cryo-EM) structure of antibody VRC44.01 in complex with the Env trimer reveals binding to the same gp120-gp41 interface site of vulnerability as antibody 35O22 from a different HIV-1-infected donor. In addition to having similar angles of approach and extensive contacts with glycans N88 and N625, VRC44 and 35O22 derive from the same IGHV1-18 gene and share convergent mutations, indicating these two antibodies to be members of the only known highly glycan-dependent multidonor class. Strikingly, both lineages achieved almost 100% neutralization breadth against virus strains displaying high-mannose glycans. The high breadth and reproducible elicitation of VRC44 and 35O22 lineages validate germline-based methods of immunogen design for targeting the HIV-1 gp120-gp41 interface. PubMed: 39675002DOI: 10.1016/j.celrep.2024.115010 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.83 Å) |
Structure validation
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