9BE1

CG-centric NF-kappaB RelA binding DNA

9BE1 の概要

• エントリーDOI: 10.2210/pdb9be1/pdb
• 分子名称: DNA (5'-D(*CP*GP*GP*GP*AP*AP*TP*TP*TP*CP*CP*GP*CP*GP*GP*AP*AP*AP*TP*TP*CP*CP*CP*G)-3'), ZINC ION, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: kappab dna, promoter, transcription, rela, dna
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 95012.80

構造登録者

Biswas, T.,Shahabi, S.,Tsodikov, O.V.,Wang, V.,Ghosh, G. (登録日: 2024-04-13, 公開日: 2024-08-07, 最終更新日: 2024-08-21)

主引用文献

Li, T.,Shahabi, S.,Biswas, T.,Tsodikov, O.V.,Pan, W.,Huang, D.B.,Wang, V.Y.,Wang, Y.,Ghosh, G.
Transient interactions modulate the affinity of NF-kappa B transcription factors for DNA.
Proc.Natl.Acad.Sci.USA, 121:e2405555121-e2405555121, 2024

PubMed Abstract: The dimeric nuclear factor kappa B (NF-κB) transcription factors (TFs) regulate gene expression by binding to a variety of κB DNA elements with conserved G:C-rich flanking sequences enclosing a degenerate central region. Toward defining mechanistic principles of affinity regulated by degeneracy, we observed an unusual dependence of the affinity of RelA on the identity of the central base pair, which appears to be noncontacted in the complex crystal structures. The affinity of κB sites with A or T at the central position is ~10-fold higher than with G or C. The crystal structures of neither the complexes nor the free κB DNAs could explain the differences in affinity. Interestingly, differential dynamics of several residues were revealed in molecular dynamics simulation studies, where simulation replicates totaling 148 μs were performed on NF-κB:DNA complexes and free κB DNAs. Notably, Arg187 and Arg124 exhibited selectivity in transient interactions that orchestrated a complex interplay among several DNA-interacting residues in the central region. Binding and simulation studies with mutants supported these observations of transient interactions dictating specificity. In combination with published reports, this work provides insights into the nuanced mechanisms governing the discriminatory binding of NF-κB family TFs to κB DNA elements and sheds light on cancer pathogenesis of cRel, a close homolog of RelA.

PubMed: 38805268
DOI: 10.1073/pnas.2405555121

実験手法

X-RAY DIFFRACTION (2.6 Å)