9AZ7

Chloride Sites in Photoactive Yellow Protein

Summary for 9AZ7

• Entry DOI: 10.2210/pdb9az7/pdb
• Descriptor: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: photoreceptor, light sensor, chromophore, photoreceptor protein, receptor, sensory transduction, signaling protein, pas, lov
• Biological source: Halorhodospira halophila
• Total number of polymer chains: 1
• Total formula weight: 14159.09

Authors

Dyda, F.,Schotte, F.,Anfinrud, P.,Cho, H.S. (deposition date: 2024-03-10, release date: 2024-03-20, Last modification date: 2024-04-24)

Primary citation

Schotte, F.,Cho, H.S.,Dyda, F.,Anfinrud, P.
Watching a signaling protein function: What has been learned over four decades of time-resolved studies of photoactive yellow protein.
Struct Dyn., 11:021303-021303, 2024

PubMed Abstract: Photoactive yellow protein (PYP) is a signaling protein whose internal p-coumaric acid chromophore undergoes reversible, light-induced -to- isomerization, which triggers a sequence of structural changes that ultimately lead to a signaling state. Since its discovery nearly 40 years ago, PYP has attracted much interest and has become one of the most extensively studied proteins found in nature. The method of time-resolved crystallography, pioneered by Keith Moffat, has successfully characterized intermediates in the PYP photocycle at near atomic resolution over 12 decades of time down to the sub-picosecond time scale, allowing one to stitch together a movie and literally watch a protein as it functions. But how close to reality is this movie? To address this question, results from numerous complementary time-resolved techniques including x-ray crystallography, x-ray scattering, and spectroscopy are discussed. Emerging from spectroscopic studies is a general consensus that three time constants are required to model the excited state relaxation, with a highly strained ground-state intermediate formed in less than 2.4 ps. Persistent strain drives the sequence of structural transitions that ultimately produce the signaling state. Crystal packing forces produce a restoring force that slows somewhat the rates of interconversion between the intermediates. Moreover, the solvent composition surrounding PYP can influence the number and structures of intermediates as well as the rates at which they interconvert. When chloride is present, the PYP photocycle in a crystal closely tracks that in solution, which suggests the epic movie of the PYP photocycle is indeed based in reality.

PubMed: 38595979
DOI: 10.1063/4.0000241

Experimental method

X-RAY DIFFRACTION (2 Å)