9AR5

Structure of Acidothermus cellulolyticus Cas9 bound with methylated DNA

Summary for 9AR5

Entry DOI	10.2210/pdb9ar5/pdb
EMDB information	43770
Descriptor	CRISPR-associated endonuclease, Csn1 family, RNA (92-MER), DNA (25-MER), ... (4 entities in total)
Functional Keywords	crispr-cas9, methylated dna, acecas9, hnh rotation, dna binding protein-dna-rna complex, dna binding protein/dna/rna
Biological source	Acidothermus cellulolyticus More
Total number of polymer chains	4
Total formula weight	172765.61
Authors	Das, A.,Rai, J.,Wang, B.,Li, H. (deposition date: 2024-02-22, release date: 2025-02-26, Last modification date: 2026-04-15)
Primary citation	Roth, M.O.,Shu, Y.,Zhao, Y.,Trasanidou, D.,Hoffman, R.D.,Trasanidis, N.,Gelasco, M.K.,Medina, M.L.,Das, D.,Rai, J.,Wang, B.,Li, H. Molecular basis for methylation-sensitive editing by Cas9 Nature, 2026 Cited by DOI: 10.1038/s41586-026-10384-z PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.88 Å)

Structure validation