9MU7
Structure of a native Drosophila melanogaster Pol II Elongation Complex with a well-defined Rpb4/Rpb7 stalk
Summary for 9MU7
| Entry DOI | 10.2210/pdb9mu7/pdb |
| EMDB information | 48622 |
| Descriptor | DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPB11, DNA-directed RNA polymerase II subunit RPB12, ... (16 entities in total) |
| Functional Keywords | polymerase, pol ii, transcription, mrna |
| Biological source | Drosophila melanogaster (fruit fly) More |
| Total number of polymer chains | 15 |
| Total formula weight | 486713.63 |
| Authors | Venette-Smith, N.L.,Vishwakarma, R.K.,Dollinger, R.,Schultz, J.,Venkatakrishnan, V.,Babitzke, P.,Anand, G.,Gilmour, D.S.,Armache, J.-P.,Murakami, K.S. (deposition date: 2025-01-13, release date: 2026-01-07, Last modification date: 2026-09-23) |
| Primary citation | Venette-Smith, N.L.,Vishwakarma, R.K.,Venkatakrishnan, V.,Dollinger, R.,Schultz, J.,Babitzke, P.,Anand, G.,Gilmour, D.S.,Armache, J.P.,Murakami, K.S. Structural Characterization of Native RNA Polymerase II Transcription Complexes and Nucleosomes in Drosophila melanogaster. Nat Commun, 17:-, 2026 Cited by PubMed Abstract: Structural studies of eukaryotic RNA polymerase II (Pol II) transcription often rely on in vitro assembly, which may not fully represent native conditions. To investigate Pol II transcription in metazoan cells, we developed a method to isolate native transcription complexes from Drosophila melanogaster embryos using FLAG-tag affinity purification and Micrococcal Nuclease treatment. Cryo-EM and proteomics studies revealed diverse transcription complexes and nucleosomes, including a metazoan Rpb4/Rpb7 stalk-less Pol II elongation complex and a hexameric nucleosome lacking an H2A/H2B dimer. Notably, nucleosome is found only downstream of the nucleosome elongation complex, underscoring it as a major energy barrier and a time-consuming step during Pol II progression through chromatin. Proteomics identified co-purified factors involved in transcription initiation, elongation, and RNA modification. This study provides a framework for investigations of transcription in cells, paving the way for future studies of transient and minor complexes. PubMed: 42711332DOI: 10.1038/s41467-026-75963-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.44 Å) |
Structure validation
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