8ZZ0

Bacterial flagellar sodium-driven stator PomA5PomB2(D24N) with 100 mM KCl

Summary for 8ZZ0

• Entry DOI: 10.2210/pdb8zz0/pdb
• EMDB information: 60585
• Descriptor: PomB, Chemotaxis protein PomA (3 entities in total)
• Functional Keywords: bacterial flagellar stator unit, sodium ion transport, membrane protein complex, membrane protein
• Biological source: Vibrio alginolyticus; More
• Total number of polymer chains: 7
• Total formula weight: 209073.26

Authors

Nishikino, T.,Takekawa, N.,Imada, K. (deposition date: 2024-06-18, release date: 2025-01-01, Last modification date: 2025-01-22)

Primary citation

Nishikino, T.,Takekawa, N.,Kishikawa, J.I.,Hirose, M.,Kojima, S.,Homma, M.,Kato, T.,Imada, K.
Structural insight into sodium ion pathway in the bacterial flagellar stator from marine Vibrio.
Proc.Natl.Acad.Sci.USA, 122:e2415713122-e2415713122, 2025

PubMed Abstract: Many bacteria swim in liquid or swarm on surface using the flagellum rotated by a motor driven by specific ion flow. The motor consists of the rotor and stator, and the stator converts the energy of ion flow to mechanical rotation. However, the ion pathway and the mechanism of stator rotation coupled with specific ion flow are still obscure. Here, we determined the structures of the sodium-driven stator of , namely PomAB, in the presence and absence of sodium ions and the structure with its specific inhibitor, phenamil, by cryo-electron microscopy. The structures and following functional analysis revealed the sodium ion pathway, the mechanism of ion selectivity, and the inhibition mechanism by phenamil. We propose a model of sodium ion flow coupled with the stator rotation based on the structures. This work provides insights into the molecular mechanisms of ion specificity and conversion of the electrochemical potential into mechanical functions.

PubMed: 39793043
DOI: 10.1073/pnas.2415713122

Experimental method

ELECTRON MICROSCOPY (3.43 Å)