8ZW9

RPS4-TIR induced At EDS1-PAD4-ADR1 heterotrimer

This is a non-PDB format compatible entry.

Summary for 8ZW9

• Entry DOI: 10.2210/pdb8zw9/pdb
• EMDB information: 60520
• Descriptor: Disease resistance protein ADR1, Isoform 1 of Protein EDS1, PAD4, ... (4 entities in total)
• Functional Keywords: effector-triggered immunity (eti), tnl signaling pathway, eds1, pad4, adr1, plant protein
• Biological source: Arabidopsis thaliana (thale cress); More
• Total number of polymer chains: 3
• Total formula weight: 223730.01

Authors

Xu, W.Y.,Zhang, Y.,Yu, H.,Wan, L. (deposition date: 2024-06-12, release date: 2024-11-06, Last modification date: 2025-01-01)

Primary citation

Yu, H.,Xu, W.,Chen, S.,Wu, X.,Rao, W.,Liu, X.,Xu, X.,Chen, J.,Nishimura, M.T.,Zhang, Y.,Wan, L.
Activation of a helper NLR by plant and bacterial TIR immune signaling.
Science, 386:1413-1420, 2024

PubMed Abstract: Plant intracellular nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain sense pathogen effectors to initiate immune signaling. TIR domains across different kingdoms possess NADase activities and can produce phosphoribosyl adenosine monophosphate/diphosphate (pRib-AMP/ADP) or cyclic ADPR (cADPR) isomers. Lipase-like proteins, EDS1 and PAD4, transduce immune signals from sensor TIR-NLRs to a helper NLR called ADR1, which executes immune function. We report the structure and function of EDS1-PAD4-ADR1 (EPA) heterotrimer in complex with pRib-AMP/ADP activated by plant or bacterial TIR signaling. 2'cADPR can be hydrolyzed into pRib-AMP and thus activates EPA signaling. Bacterial TIR domains producing 2'cADPR also activate EPA function. Our findings suggest that 2'cADPR may be the storage form of the unstable signaling molecule pRib-AMP.

PubMed: 39509471
DOI: 10.1126/science.adr3150

Experimental method

ELECTRON MICROSCOPY (3.03 Å)