8ZVC

ShosT with PRPP-Mg

Summary for 8ZVC

• Entry DOI: 10.2210/pdb8zvc/pdb
• Descriptor: ShosT, 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (7 entities in total)
• Functional Keywords: shost, toxin, immune system
• Biological source: Escherichia coli O1:K1 / APEC
• Total number of polymer chains: 1
• Total formula weight: 50078.57

Authors

Yu, Y.,Chen, Q.,Pu, H. (deposition date: 2024-06-11, release date: 2025-04-09, Last modification date: 2025-04-16)

Primary citation

Pu, H.,Chen, Y.,Zhao, X.,Dai, L.,Tong, A.,Tang, D.,Chen, Q.,Yu, Y.
A toxin-antitoxin system provides phage defense via DNA damage and repair.
Nat Commun, 16:3141-3141, 2025

PubMed Abstract: Widespread in bacteria and archaea, toxin-antitoxin (TA) systems have been recently demonstrated to function in phage defense. Here we characterize the anti-phage function of a type IV TA system, ShosTA. Using structural and biochemical approaches, we show that ShosT couples phosphoribosyltransferase and pyrophosphatase activities to disrupt purine metabolism, resulting in DNA duplication, cell filamentation and ultimate cell death. ShosA binds DNA and likely recruits other proteins to facilitate DNA homologous recombination to antagonize ShosT's toxicity. We identify Gp0.7 of T7 phage as a trigger for ShosTA system via shutting off the protein synthesis, and the C-terminus-mediated intrinsic instability of ShosA releases the toxicity of the existing ShosT proteins. Collectively, our results provide a novel toxin-antitoxin mechanism for anti-phage immunity and shed light on the triggering of this TA system.

PubMed: 40169592
DOI: 10.1038/s41467-025-58540-9

Experimental method

X-RAY DIFFRACTION (1.8 Å)