8ZTY
Cryo-EM structure of the Cas13a-rAcrVIA1 complex
Summary for 8ZTY
| Entry DOI | 10.2210/pdb8zty/pdb |
| EMDB information | 60476 |
| Descriptor | CRISPR-associated endoribonuclease Cas13a, rAcrVIA1 (2 entities in total) |
| Functional Keywords | rna binding protein/rna, structural protein, rna binding protein-rna complex |
| Biological source | Listeria seeligeri serovar 1/2b str. SLCC3954 More |
| Total number of polymer chains | 2 |
| Total formula weight | 153223.68 |
| Authors | Zhang, J.T.,Li, Y.L.,Jia, N. (deposition date: 2024-06-07, release date: 2025-04-23, Last modification date: 2025-05-07) |
| Primary citation | Hayes, V.M.,Zhang, J.T.,Katz, M.A.,Li, Y.,Kocsis, B.,Brinkley, D.M.,Jia, N.,Meeske, A.J. RNA-mediated CRISPR-Cas13 inhibition through crRNA structural mimicry. Science, 388:387-391, 2025 Cited by PubMed Abstract: To circumvent CRISPR-Cas immunity, phages express anti-CRISPR factors that inhibit the expression or activities of Cas proteins. Whereas most anti-CRISPRs described to date are proteins, recently described small RNAs called RNA anti-CRISPRs (rAcrs) have sequence homology to CRISPR RNAs (crRNAs) and displace them from cognate Cas nucleases. In this work, we report the discovery of rAcrVIA1-a plasmid-encoded small RNA that inhibits the RNA-targeting CRISPR-Cas13 system in its natural host, . We solved the cryo-electron microscopy structure of the Cas13-rAcr complex, which revealed that rAcrVIA1 adopts a fold nearly identical to crRNA despite sharing negligible sequence similarity. Collectively, our findings expand the diversity of rAcrs and reveal an example of immune antagonism through RNA structural mimicry. PubMed: 40273258DOI: 10.1126/science.adr3656 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.85 Å) |
Structure validation
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