8ZQ3

Structure of ORP1L-RAB7A in the presence of GDP

Summary for 8ZQ3

• Entry DOI: 10.2210/pdb8zq3/pdb
• Descriptor: Ras-related protein Rab-7a, Oxysterol-binding protein-related protein 1, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: oxysterol binding protein (osbp) the human osbp-related gene/protein (orp) family, endocytosis
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 46701.81

Authors

Lu, Q.,Zhang, J.,Xu, C. (deposition date: 2024-05-31, release date: 2024-06-26)

Primary citation

Lu, Q.,Zhu, Z.,Zhang, J.,Xu, C.
Structure of GDP-bound Rab7 Q67L in complex with ORP1L.
Biochem.Biophys.Res.Commun., 725:150232-150232, 2024

PubMed Abstract: Molecular processes are orchestrated by various proteins that promote early endosomes to become late endosomes and eventually fuse with lysosomes, guaranteeing the degradation of the content. Rab7, which is localized to late endosomes, is one of the most well-known GTPases. ORP1L is recruited by Rab7 to facilitate the fusion of late endosomes and lysosomes. Here, we present the structure of GDP-bound Rab7 Q67L with ORP1L. Structural analysis, supported by biochemical and ITC binding experiments, not only provides structural insight into the interactions between the ORP1L ANK domain and Rab7 but also suggests that the GTPase activity of Rab7 does not interfere with its ORP1L-binding capacity.

PubMed: 38897042
DOI: 10.1016/j.bbrc.2024.150232

Experimental method

X-RAY DIFFRACTION (2.43 Å)