8ZL2
Cryo-EM structure of the Drosophila INDY (DIDS-bound asymmetric, pH 6)
Summary for 8ZL2
| Entry DOI | 10.2210/pdb8zl2/pdb |
| EMDB information | 60216 |
| Descriptor | Protein I'm not dead yet, 4,4'-Diisothiocyano-2,2'-stilbenedisulfonic acid, PHOSPHATIDYLETHANOLAMINE, ... (4 entities in total) |
| Functional Keywords | indy, slc13a5, citrate transporter, membrane protein, dids |
| Biological source | Drosophila melanogaster (fruit fly) |
| Total number of polymer chains | 2 |
| Total formula weight | 133639.06 |
| Authors | Kim, S.,Park, J.G.,Choi, S.H.,Kim, J.W.,Jin, M.S. (deposition date: 2024-05-17, release date: 2025-01-29, Last modification date: 2025-08-13) |
| Primary citation | Kim, S.,Park, J.G.,Choi, S.H.,Kim, J.W.,Jin, M.S. Cryo-EM structures reveal the H + /citrate symport mechanism of Drosophila INDY. Life Sci Alliance, 8:-, 2025 Cited by PubMed Abstract: I'm Not Dead Yet (INDY) functions as a transporter for citrate, a key metabolite in the citric acid cycle, across the plasma membrane. Partial deficiency of INDY extends lifespan, akin to the effects of caloric restriction. In this work, we use cryo-electron microscopy to determine structures of INDY in the presence and absence of citrate and in complex with the well-known inhibitor 4,4'-diisothiocyano-2,2'-disulfonic acid stilbene (DIDS) at resolutions ranging from 2.7 to 3.6 Å. Together with functional data obtained in vitro, the INDY structures reveal the H/citrate co-transport mechanism, in which aromatic residue F119 serves as a one-gate element. They also provide insight into how protein-lipid interactions at the dimerization interface affect the stability and function of the transporter, and how DIDS disrupts the transport cycle. PubMed: 39884835DOI: 10.26508/lsa.202402992 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
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