8ZF0

pRib-ADP bound OsEDS1-PAD4-ADR1 complex

This is a non-PDB format compatible entry.

Summary for 8ZF0

• Entry DOI: 10.2210/pdb8zf0/pdb
• EMDB information: 60049
• Descriptor: RPW8 domain-containing protein, EDS1, Lipase-like PAD4, ... (4 entities in total)
• Functional Keywords: adr1, eds1, pad4, plant immunity, prib-adp, nlr, plant protein
• Biological source: Oryza sativa Japonica Group (Japanese rice); More
• Total number of polymer chains: 3
• Total formula weight: 236704.33

Authors

Xu, W.Y.,Zhang, Y. (deposition date: 2024-05-07, release date: 2024-11-13, Last modification date: 2025-01-01)

Primary citation

Wu, Y.,Xu, W.,Zhao, G.,Lei, Z.,Li, K.,Liu, J.,Huang, S.,Wang, J.,Zhong, X.,Yin, X.,Wang, Y.,Zhang, H.,He, Y.,Ye, Z.,Meng, Y.,Chang, X.,Lin, H.,Wang, X.,Gao, Y.,Chai, J.,Parker, J.E.,Deng, Y.,Zhang, Y.,Gao, M.,He, Z.
A canonical protein complex controls immune homeostasis and multipathogen resistance.
Science, 386:1405-1412, 2024

PubMed Abstract: The calcium (Ca) sensor ROD1 (RESISTANCE OF RICE TO DISEASES1) is a master regulator of immunity in rice. By screening suppressors of mutants, we show that ROD1 governs immune homeostasis by surveilling the activation of a canonical immune pathway. Mutations in (), (), (), and () all abolish enhanced disease resistance of plants. OsTIR catalyzes the production of second messengers 2'-(5″-phosphoribosyl)-5'-adenosine monophosphate (pRib-AMP) and diphosphate (pRib-ADP), which trigger formation of an OsEDS1-OsPAD4-OsADR1 (EPA) immune complex. ROD1 interacts with OsTIR and inhibits its enzymatic activity, whereas mutation of leads to constitutive activation of the EPA complex. Thus, we unveil an immune network that fine-tunes immune homeostasis and multipathogen resistance in rice.

PubMed: 39509474
DOI: 10.1126/science.adr2138

Experimental method

ELECTRON MICROSCOPY (2.96 Å)