8ZDX

Crystal structure of MjHKU4r-CoV-1 RBD bound to hDPP4

8ZDX の概要

• エントリーDOI: 10.2210/pdb8zdx/pdb
• 分子名称: Dipeptidyl peptidase 4 membrane form, Spike glycoprotein, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: complex of a pangolin coronavirus spike protein bound to receptor, viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 219207.61

構造登録者

Yang, M.,Li, Z.,Xu, Y.,Zhang, S. (登録日: 2024-05-03, 公開日: 2024-10-30, 最終更新日: 2025-05-14)

主引用文献

Yang, M.,Li, Z.,Chen, J.,Li, Y.,Xu, R.,Wang, M.,Xu, Y.,Chen, R.,Ji, W.,Li, X.,Wei, J.,Zhou, Z.,Ren, M.,Ma, K.,Guan, J.,Mo, G.,Zhou, P.,Shu, B.,Guo, J.,Yuan, Y.,Shi, Z.L.,Zhang, S.
Structural basis for human DPP4 receptor recognition by a pangolin MERS-like coronavirus.
Plos Pathog., 20:e1012695-e1012695, 2024

PubMed Abstract: Middle East respiratory syndrome coronavirus (MERS-CoV) and the pangolin MERS-like coronavirus MjHKU4r-CoV-1 employ dipeptidyl peptidase 4 (DPP4) as an entry receptor. MjHKU4r-CoV-1 could infect transgenic mice expressing human DPP4. To understand the mechanism of MjHKU4r-CoV-1 entry into cells, we determined the crystal structures of the receptor binding domain (RBD) of MjHKU4r-CoV-1 spike protein bound to human DPP4 (hDPP4) and Malayan pangolin DPP4 (MjDPP4), respectively. The overall hDPP4-binding mode of MjHKU4r-CoV-1 RBD is similar to that of MERS-CoV RBD. MjHKU4r-CoV-1 RBD shows higher binding affinity to hDPP4 compared to the bat MERS-like coronavirus Ty-BatCoV-HKU4. Via swapping residues between MjHKU4r-CoV-1 RBD and Ty-BatCoV-HKU4 RBD, we identified critical determinants on MjHKU4r-CoV-1 that are responsible for virus usage of hDPP4. Our study suggests that MjHKU4r-CoV-1 is more adapted to the human receptor compared to the bat HKU4 coronavirus and highlights the potential of virus emergence into the human population.

PubMed: 39514585
DOI: 10.1371/journal.ppat.1012695

実験手法

X-RAY DIFFRACTION (2.6 Å)