8ZDJ
Cryo-EM structure of Mycobacteriophage Douge genome-packed connector (gp5, gp9, gp10, gp12 and gp13)
Summary for 8ZDJ
| Entry DOI | 10.2210/pdb8zdj/pdb |
| EMDB information | 39983 39985 39986 39988 60714 |
| Descriptor | Portal Protein (gp5), Stopper Protein (gp10), Tail Tube Protein (gp13), ... (5 entities in total) |
| Functional Keywords | mycobacteriophage, phage structure, icosahedral, cryo-em, protein assembly, virus, viral protein |
| Biological source | Mycolicibacterium smegmatis MC2 155 More |
| Total number of polymer chains | 42 |
| Total formula weight | 1241947.55 |
| Authors | Maharana, J.,Wang, C.H.,Tsai, L.A.,Lowary, T.L.,Ho, M.C. (deposition date: 2024-05-02, release date: 2025-06-25, Last modification date: 2025-09-03) |
| Primary citation | Maharana, J.,Wang, C.H.,Tsai, L.A.,Liao, Y.T.,Yang, C.H.,Shen, M.C.,Macale, L.S.,Tran, T.N.,Narsico, J.,Perez, R.J.,Tewary, S.K.,Wu, J.L.,Lin, H.Y.,Chang, S.W.,Franklin, A.,Moynihan, P.J.,Jacobs-Sera, D.,Freeman, K.G.,Hatfull, G.F.,Lowary, T.L.,Ho, M.C. Cryo-EM and cryo-ET reveal the molecular architecture and host interactions of mycobacteriophage Douge. Cell Rep, 44:116057-116057, 2025 Cited by PubMed Abstract: Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions could allow structure-guided phage engineering to increase efficacy and broaden host range, but such information is currently unavailable. We describe the cryoelectron microscopy (cryo-EM) structure of mycobacteriophage Douge, which contains 1,105 protein subunits assembled into a complete siphophage and is coated with glycan-binding domains for mycobacterial cell surface interactions. When filled with viral genome, the channel spanning the connector, tail, and baseplate is sealed by tape measure proteins, providing a genome gating system and requiring limited structural changes for genome ejection upon phage-host contact. Nanometer-resolution cryoelectron tomography (cryo-ET) snapshots of phage-host interactions show that the baseplate remains attached to the mycobacterial outer membrane during viral genome ejection. This study reveals high-resolution structural details of this mycobacteriophage and its interaction with host glycans. PubMed: 40711877DOI: 10.1016/j.celrep.2025.116057 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.74 Å) |
Structure validation
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