8ZCY

Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in an interrogation state

Summary for 8ZCY

Entry DOI	10.2210/pdb8zcy/pdb
EMDB information	39941
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA, Non-target DNA strand, ... (4 entities in total)
Functional Keywords	crispr-cas9, genome engineering, hydrolase-rna-dna complex, dna binding protein
Biological source	Staphylococcus aureus More
Total number of polymer chains	4
Total formula weight	182899.76
Authors	Omura, S.N.,Nakagawa, R.,Yamashita, K.,Nishimasu, H.,Nureki, O. (deposition date: 2024-04-30, release date: 2025-11-05, Last modification date: 2026-04-15)
Primary citation	Omura, S.N.,Nakagawa, R.,Kajimoto, S.,Okazaki, S.,Ishiguro, S.,Mori, H.,Onishi, K.,Kashiwakura, Y.,Hiramoto, T.,Hirano, H.,Yamashita, K.,Jividen, K.,Tsai, S.Q.,Yachie, N.,Ohmori, T.,Nishimasu, H.,Nureki, O. Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation. Nat Commun, 2026 Cited by DOI: 10.1038/s41467-026-71626-2 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.17 Å)

Structure validation