8ZCY

Cryo-EM structure of eSaCas9_NNG-guide RNA-target DNA complex in an interrogation state

Summary for 8ZCY

• Entry DOI: 10.2210/pdb8zcy/pdb
• EMDB information: 39941
• Descriptor: CRISPR-associated endonuclease Cas9, sgRNA, Non-target DNA strand, ... (4 entities in total)
• Functional Keywords: crispr-cas9, genome engineering, hydrolase-rna-dna complex, dna binding protein
• Biological source: Staphylococcus aureus; More
• Total number of polymer chains: 4
• Total formula weight: 182899.76

Authors

Omura, S.N.,Nakagawa, R.,Yamashita, K.,Nishimasu, H.,Nureki, O. (deposition date: 2024-04-30, release date: 2025-11-05)

• Primary citation: Omura, S.N.,Nakagawa, R.,Kajimoto, S.,Okazaki, S.,Ishiguro, S.,Mori, H.,Kashiwakura, Y.,Hiramoto, T.,Hirano, H.,Yamashita, K.,Jividen, K.,Tsai, S.Q.,Yachie, N.,Ohmori, T.,Nishimasu, H.,Nureki, O.; Molecular engineering and structural mechanism of the compact CRISPR-Cas9 nuclease; To Be Published,

Experimental method

ELECTRON MICROSCOPY (3.17 Å)