8ZB9

CryoEM structure of non-structural protein 1 dimer from Yellow Fever Virus

Summary for 8ZB9

• Entry DOI: 10.2210/pdb8zb9/pdb
• EMDB information: 39898
• Descriptor: Non-structural protein 1 (1 entity in total)
• Functional Keywords: flavivirus, non-structural protein 1, viral protein
• Biological source: Yellow fever virus 17D
• Total number of polymer chains: 2
• Total formula weight: 81116.90

Authors

Pan, Q.,Chen, Q.,Hu, H.L. (deposition date: 2024-04-26, release date: 2024-09-11, Last modification date: 2024-11-13)

Primary citation

Pan, Q.,Chen, Q.,Zhang, W.,Jiao, H.,Yu, L.,Hu, H.
Structural Insights into the Dynamic Assembly of a YFV sNS1 Tetramer.
Viruses, 16:-, 2024

PubMed Abstract: Yellow fever virus (YFV) infections can cause severe diseases in humans, resulting in mass casualties in Africa and the Americas each year. Secretory NS1 (sNS1) is thought to be used as a diagnostic marker of flavivirus infections, playing an essential role in the flavivirus life cycle, but little is known about the composition and structure of YFV sNS1. Here, we present that the recombinant YFV sNS1 exists in a heterogeneous mixture of oligomerizations, predominantly in the tetrameric form. The cryoEM structures show that the YFV tetramer of sNS1 is stacked by the hydrophobic interaction between β-roll domains and greasy fingers. According to the 3D variability analysis, the tetramer is in a semi-stable state that may contain multiple conformations with dynamic changes. We believe that our study provides critical insights into the oligomerization of NS1 and will aid the development of NS1-based diagnoses and therapies.

PubMed: 39205186
DOI: 10.3390/v16081212

Experimental method

ELECTRON MICROSCOPY (3.31 Å)