8ZAP

ExoC110T class 1 channelrhodopsin

Summary for 8ZAP

• Entry DOI: 10.2210/pdb8zap/pdb
• EMDB information: 39883
• Descriptor: C110T-class1, POTASSIUM ION, RETINAL, ... (4 entities in total)
• Functional Keywords: channelrhodopsin, membrane protein
• Biological source: Hyphochytrium catenoides
• Total number of polymer chains: 3
• Total formula weight: 91356.49

Authors

Zhang, M.F. (deposition date: 2024-04-25, release date: 2024-09-04, Last modification date: 2024-10-23)

Primary citation

Shan, Y.,Zhao, L.,Chen, M.,Li, X.,Zhang, M.,Pei, D.
Channelrhodopsins with distinct chromophores and binding patterns.
Nat Commun, 15:7292-, 2024

PubMed Abstract: Channelrhodopsins are popular optogenetic tools in neuroscience, but remain poorly understood mechanistically. Here we report the cryo-EM structures of channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii and H. catenoides kalium channelrhodopsin (KCR1). We show that ChR2 recruits an endogenous N-retinylidene-PE-like molecule to a previously unidentified lateral retinal binding pocket, exhibiting a reduced light response in HEK293 cells. In contrast, H. catenoides kalium channelrhodopsin (KCR1) binds an endogenous retinal in its canonical retinal binding pocket under identical condition. However, exogenous ATR reduces the photocurrent magnitude of wild type KCR1 and also inhibits its leaky mutant C110T. Our results uncover diverse retinal chromophores with distinct binding patterns for channelrhodopsins in mammalian cells, which may further inspire next generation optogenetics for complex tasks such as cell fate control.

PubMed: 39181878
DOI: 10.1073/pnas.1818707116

Experimental method

ELECTRON MICROSCOPY (2.6 Å)